Microfluidics: Advances in Microfluidics‐Based Technologies for Single Cell Culture (Adv. Biosys. 11/2019)

Advances in Microfluidics and Lab-on-a-Chip Technologies

Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains like biology research, infectious disease management, food safety and bio-defense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care (POC). They have also introduced new functionality, enhanced the sens...

Microfluidics for single-cell genetic analysis.

The ability to correlate single-cell genetic information to cellular phenotypes will provide the kind of detailed insight into human physiology and disease pathways that is not possible to infer from bulk cell analysis. Microfluidic technologies are attractive for single-cell manipulation due to precise handling and low risk of contamination. Additionally, microfluidic single-cell techniques ca...

Microengineered Heterogeneous Substrates for Cell Culture by Electro-microfluidics

Cultivating cells on a heterogeneous substrate prepared by assembly of hydrogels and arrangement of microparticles using electric manners on an electro-microfluidic (EMF) device are reported. We demonstrated a multifunctional integrated EMF device, combining (1) electrical manipulations of dielectrophoresis (DEP) and electrowetting-on-dielectric (EWOD) and (2) UV crosslinking technique, in orde...

Technologies for Single-Cell Isolation

The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to iden...

Emerging Tools for Single-cell Analysis Advances in Optical Measurement Technologies